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Delphi-X Inverso fluorescence
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Informations
Delphi-X Inverso fluorescence
The Delphi-X Inverso™ for fluorescence is an inverted microscope for the professional who needs to observe tissues or cells stained with fluorescence dyes. Super contrast semi-apochromatic Fluarex™ objectives with collar adjustment ring assure perfect observation of cells or tissues in 1.2 mm thick Petri dishes or flasks
Icons are used to indicate the several functions of a microscope
Click and find more details about the meaning of each icon
Highlights
- Life science applications
- Observation of fluorescence in tissues and cells
- Brightfield, phase contrast fluorescence observations
- DIC (differential interference contrast)
- Long working distance IOS plan semi-apochromatic Fluarex achromatic objectives
- Revolving sextuple nosepiece
- 10 W LED or 100 W halogen transmitted illumination
- 100 W HBO mercury vapor fluorescence illumination
- Backwards tilting condenser arm
- Easy placement of large objects
| MODELS | LWD Plan phase 10x/20x/S40x objectives | 100 W Halogen illumination | 10 W LED illumination | 100 W HBO fluorescence illumination |
| DI.3053‑PLPHFi | • | • | • | |
| DI.3053‑PLPHFi | • | • | • |
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EYEPIECES
Pair of DIN HWF plan 10x/25 mm or 10x/22 mm eyepieces with diopter adjustment
HEAD
Trinocular 45° inclined tubes. Interpupillary distance of 47 to 78 mm. Trinocular head with 100-0, 80/20 and 0/100 positions
NOSEPIECE
Revolving sextuple nosepiece on ball-bearings. The nosepiece can be equipped with DIC prisms for the 10x/20x and 40x objectives
OBJECTIVES
High numerical infinity semi-apochromatic long working distance plan phase Fluarex PLPHFi 10x/0.30, 20x/0.45, S40x/0.60 IOS objectives enable the sub-cellular structures. An optional 60x/0.80 IOS objective is also available. The 20x, S40x and optional S60 objectives are equipped with collar adjustment ring for glass thicknesses from 0 to 1.2 mm
Fluarex™ objectives are made of low-absorption glass. Fluarex™ is a trademark of Euromex microscopen bv. All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
STAGE
Stage 340 x 230 mm, equipped with a coaxial mechanical 130 x 85 mm X-Y stage. Supplied with:
• Metal insert for O 38 mm cell culture dish
• Metal insert for O 54 mm cell culture dish or 76 x 26 mm slides
• Metal insert for Multiwell plates (127.76 x 85.48 mm)
• Metal insert for Teraski plates (83.3 x 58.0 mm)
• Optional universal cell culture holder
CONDENSER
Rotating N.A. 0.55 condenser with iris diaphragm. Working distance 26 mm. Supplied with phase contrast annuli for 10x/20x, 40x and 60x objectives and the DIC prisms for the 10x/20x/40x and 60x objectives. Three filter holders allow the insertion of Ø 38 mm filters in the optical path. The arm with condenser can be tilted backwards for convenient access to the stage. This allows easy placement of large objects like flasks and Petri dishes
LENS CHANGER
A lens changer with a 1.5x lens allow intermediary magnifications of 1.5 times the standard objective magnifications. Switching from 20x to 30x or 40 to 60 and from 60x to 90x magnification can be done without the need to re-focus on the sample
6-Position fluorescence filter set wheel and 1.5 lens changer
FLUORESCENCE ATTACHMENT
The Delphi-X Inverso for fluorescence microscopy is supplied with a 6-position fluorescence filter set wheel and comes with two fluorescence filter sets for blue and green excitation (DI.9745 and DI.9746). Other filter sets are optional
DI.9745 Blue excitation, EX 460-495 DM 505 EM 510 long pass
DI.9746 Green excitation, EX 510-550 DM 570 EM 575 long pass
DI.9747 Violet excitation, EX 400-410 DM 455 EM 460 Long pass
DI.9748 Ultra violet excitation, EX 330-385 DM 410 EM 420 Long pass
DI.9750.TRITC Green excitation, EX 528-553 DM 565 EM 578-633 band pass
DI.9750.FITC Blue excitation, EX 460-495 DM 505 EM 510-550 band pass
DI.9750.DAPI UV excitation, EX 360-390 DM 415 EM 435-485 band pass
DI.9750.R Red excitation, EX 620-650 DM 660 EM 670-750 band pass
FOCUSING ADJUSTMENT
Coaxial 10 mm coarse and fine adjustments, 1 μm precision and with friction adjustment
ILLUMINATION
Fluorescence illumination with a 100 W mercury vapor lamp with external 100-240 V power supply. Transmitted 10 W LED or 100 W halogen illumination and internal power supply. Fuse holder 3.15 A / 250 V
PACKAGE CONTENT
Supplied with metal insert for Ø 38 mm cell culture dish and a metal insert for Ø 54 mm cell culture dish with 76 x 26 mm slides, metal insert for Multiwell plates, metal insert for Teraski plates, blue and green interference Ø 45 mm filters, power cord, dust cover, green filter and alignment telescope and user manual
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DI.6010 Super wide field SWF 10x/22 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube |
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DI.6210 Super wide field SWF 10x/22 mm focusable eyepiece for Delphi-X Inverso, 30 mm diameter tube |
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DI.6012 Wide field WF 12.5x/17.5 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso |
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DI.6015 Wide field WF 15x/16 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso |
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DI.6020 Wide field WF 20x/12 mm eyepiece for Ø 30 mm tube for Delphi-X Inverso |
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DI.6099-L Eyeshade for eyepieces |
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DI.8704 Infinity Plan Semi-Apochromatic Phase contrast PLPHFi-Apo 4x/0.13 objective, working distance 16,8 mm. Cover glass correction 1.2 mm |
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DI.8710 Infinity Plan Semi-Apochromatic Phase contrast PLPHFi-Apo 10x/0.30 objective. Working distance 10.2 mm. Cover glass correction 1.2 mm |
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DI.8720 Infinity Plan Semi-Apochromatic Phase contrast PLPHFi-Apo 20x/0.45 objective with collar ring for adjustment of working distance between 7.5 to 8.8 mm. Cover glass correction 1.2 mm |
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DI.8740 Infinity Plan Semi-Apochromatic Phase contrast PLPHFi-Apo 40x/0.60 objective with collar ring for adjustment of working distance between 3 to 4.4 mm. Cover glass correction 1.2 mm |
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DI.9690-10 DIC prism for rotating disc condenser for Life Science Delphi Inverso models. To be used with Plan Semi-Apo 10x objective |
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DI.9690-20 DIC prism for rotating disc condenser for Life Science Delphi Inverso models. To be used with Plan Semi-Apo 20x objective |
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DI.9690-40 DIC prism for rotating disc condenser for Life Science Delphi Inverso models. To be used with Plan Semi-Apo 40x objective |
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DI.9695 DIC polarization filter for transmitted light |
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DI.9200-6 Fluorescence attachment, 6-positions, UV shield; 100 W HBO mercury vapor lamp, with collimating lens, with external PSU BUT without objectives, without filter sets and without empty cubes |
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DI.9745-6 Standard filter set for Blue excitation EX 460-495 nm (BP) DM 505 EM 510 nm (LP). Needs empty cube |
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DI.9746-6 Standard filter set for green excitation EX 510-550 nm (BP) DM 570 EM 575 (LP) Needs empty cube |
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DI.9747-6 Standard filter set for Violet excitation EX 400-410 nm (BP) DM 455 EM 460 (LP) Needs empty cube |
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DI.9748-6 Standard filter set for UV excitation EX 330-385 nm (BP) DM 410 EM 420 (LP) Needs empty cube |
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DI.9749 Empty cube for Delphi-X Observer Fluorescence attachment |
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DI.9750.19000 Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM435 (LP) optimized for UV/DAPI, Calcofluor White. Needs empty filter block |
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DI.9750.19006 Fluorescence filter set EX560 (BP40: 540-580 nm) DM600 (LP) EM610 (LP) optimized for FM 4-64, Turbo FP650. Needs empty filter block |
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DI.9750.19008 Fluorescence filter set EX450 (BP50: 425-475 nm) DM485 (LP) EM495 (LP) optimized for Auramine Longpass. Needs empty filter block |
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DI.9750.19010 Fluorescence filter set EX480 (BP30: 465-495 nm) DM505 (LP) EM600 (LP) optimized for FM 1-43 / 4-64 , Pulsar 650 and Chlorophyll Longpass. Needs empty filter block |
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DI.9750.19011 Fluorescence filter set EX405 (BP30: 390-420 nm) DM505 (LP) EM450 (LP) optimized for Coumarin and Pacific Blue Longpass. Needs empty filter block |
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DI.9750.19012 Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM515 (LP) optimized for FluoroGold and CalcoFluor White Longpass. Needs empty filter block |
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DI.9750.39000 Fluorescence filter set EX375 (BP28: 361-389 nm) DM415 (LP) EM460 (BP50: 435-485 nm) optimized for DAPI, Hoechst, AlexaFluor 350. Needs empty filter block |
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DI.9750.39001 Fluorescence filter set EX435 (BP20: 425-445 nm) DM455 (LP) EM480 (BP30: 465-495 nm) optimized for AmCyan, Atto 425, Cerulean, CFP, ECFP fluorophore. Needs empty filter block IS.9749 |
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DI.9750.39002 Fluorescence filter set EX480 (BP30: 465-495 nm) DM505(LP) EM535 (BP40: 515-555 nm) optimized for Acridine Orange + DNA, Alexa Fluor 488™, Atto 488, Azami Green, BODIPY FL/pH7.2, Calcein, Calcium, Green™-1, Cy2™, DiO, DyLight 488, EGFP, Emerald, GFP, FAM, FITC, Fluo-4, GFP, LysoTracker Green/pH 5.2, MitoTracker Green, FM/MeOH, mWasabi, Oregon Green™ 488, SYBR® Green I, SYTO 9/DNA, ZsGreen fluorophore. Needs empty filter block |
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DI.9750.39003 Fluorescence filter set EX495 (BP20: 485-505 nm) DM515 (LP) EM540 (BP30: 525-555 nm) optimized for Alexa Fluor 514™, Citrine, EYFP/pH 7, F/lAsH-CCPFCC, mCitrine, Oregon Green™ 514, Rhodamine 123, Topaz. Needs empty filter block |
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DI.9750.39004 Fluorescence filter set EX540 (BP25: 528-553 nm) DM565 (LP) EM605 (BP55: 578-633 nm) optimized for Alexa Fluor 546™, Alexa Fluor 555™, Atto 550, CAL, Fluor® Red 590, Cy3™, DiI, DsRed, DyLight 549, MitoTracker Orange/MeOH, Resorufin, Rhod-2, TagRFP, TAMRA tdTomato, Tetramethylrhodamine, isothiocyanate, TRITC. Needs empty filter block |
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DI.9750.39005 Fluorescence filter set EX540 (BP25: 528-552 nm) DM565 (LP) EM 620 (BP60: 590-650 nm) optimized for TRITC-red-shifted, Ethidium homidimer-1/DNA, Propidium Iodide. Needs empty filter block |
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DI.9750.39007 Fluorescence filter set EX620 (BP50: 595-645 nm) DM655 (LP) EM 690 (BP50: 665-715 nm) optimized for Alexa Fluor 647™, Allophycocyanin (APC), Atto 647N, Cy5™, DiD, Draq5, DyLight 649, MitoTracker Deep Red 633/MeOH, Nile Blue, Quasar® 670, SYTO® 60, TO-PRO™-3. Needs empty filter block |
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DI.9750.39008 Fluorescence filter set EX420 (BP40: 400-440) DM455 (LP) EM 535 (BP40: 515-555) optimized for Lucifer Yellow/Tetracycline/AlexaFluor 430. Needs empty filter block |
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DI.9750.39009 Fluorescence filter set EX480 (BP30: 465-495) DM505 (LP) EM 620 (BP60: 590-650) for Acridine Orange + RNA, Di-8-ANEPPS, non-radiometric, DiA, Ethidium Bromide, FM™ 1-43, LysoTracker Yellow HCK-123, Pulsar™ 650. Needs empty filter block |
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DI.9750.39010 Fluorescence filter set EX560 (BP40: 540-580) DM600 (LP) EM635 (BP60: 615-665) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block IS.9749 |
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DI.9750.49000 Fluorescence filter set EX350 (BP50: 325-375) DM400 (LP) EM460 (BP50: 435-485) optimized for Texas Red, mCherry, AlexaFuor 594. Needs empty filter block IS.9749 |
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DI.9810 C-mount 1x adapter voor Delphi-X Inverso microscoop |
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DI.9840 C-mount adapter met hoge resolutie 0.40x objectief voor 1/3 inch C-mount camera |
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DI.9850 C-mount adapter met hoge resolutie 0.50x objectief voor 1/2 inch C-mount camera |