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iScope fluorescence
Product information
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Technical specifications
accessories
Information
For biological and medical applications fluorescence microscopes are used to observe certain parts of living cells and tissues with help of fluorophores. One or more fluophores are added to the parts of the specimen to be observed. When the fluorophores are exposed to so-called exicitation light, the excitation energy is absorbed by the fluorophore that start emitting so-called emission light that can be observed by the microscope. One can distinguish tumor cells from other cells, follow biological processes or prove the presence or absence of antibodies, etc
The excellent optics and mechanical components make the iScope a state-of-the art microscope that is suitable for fluorescence applications in Life Science and Biotechnical fields
The iScope fluorescence microscopy can be supplied with:
• 6-position rotating attachment with 100 W mercury vapor light source
• 3-position slider attachment with 100 W mercury vapor light source
• 1-position slider attachment with a 3 W NeoLED 465-475 nm blue light source
• 1-position slider attachment with a 3 W NeoLED 520-530 nm green light source
Icons are used to indicate the several functions of a microscope.
Click and find more details about the meaning of each icon
Highlights
- HWF 10x/22 mm eyepieces
- Binocular and trinocular models
- Optional ergonomic tilting head
- Quintuple revolving nosepiece
- E-plan, plan and plan Fluarex IOS corrected objectives
- 187/230 x 140 mm rackless stage with integrated X-Y stage
- Optional ceramic stage
- Diascopic intensity adjustable 3 W NeoLED Köhler illumination
- Epi-fluorescence 100 W HBo mercury vapor
- Blue and Green LED fluorescence
- iCare sensor for energy saving
- CSS - Cable Storage System
6-positions rotating attachment
With 100 W mercury vapor light source
The 6-positions rotating fluorescence attachments are supplied with a standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for excitation in the blue and green light spectrum and a UV protection shield
| MODELS | Binocular | Trinocular | WF 10x/22 mm | Diopter adjustable on left tube | Diopter adjustable on both tubes | E-plan EPLi 4x/10x/S40x and S100x objectives | Plan PLi 4x/10x/S40x and S100x objectives | Plan PLFi 4x/10x/S40 and S100x objectives | 100 W HBO light source | Filter sets (blue&green) | NeoLED illumination | |
| IS.3152‑EPLi/6 | • | • | • | • | • | • | • |  |
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| IS.3153‑EPLi/6 | • | • | • | • | • | • | • |  |
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| IS.3152‑PLi/6 | • | • | • | • | • | • | • |  |
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| IS.3153‑PLi/6 | • | • | • | • | • | • | • |  |
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| IS.3152‑PLFi/6 | • | • | • | • | • | • | • | |||||
| IS.3153‑PLFi/6 | • | • | • | • | • | • | • |
3-positions slider attachment
With 100 W mercury vapor light source
The 3-positions slider fluorescence attachments are supplied with standard 100 W HBO mercury vapor light source for broad powerful light spectrum. Supplied with standard fluorescence filters sets for blue and green excitation and a UV protection shield
| MODELS | Binocular | Trinocular | WF 10x/22 mm | Diopter adjustable on left tube | Diopter adjustable on both tubes | E-plan EPLi 4x/10x/S40x and S100x objectives | Plan PLi 4x/10x/S40x and S100x objectives | Plan PLFi 4x/10x/S40x and S100x objectives | 100 W HBO light source | Filter sets (blue&green) | NeoLED illumination | |
| IS.3152‑EPLi/3 | • | • | • | • | • | • | • |  |
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| IS.3153‑EPLi/3 | • | • | • | • | • | • | • |  |
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| IS.3152‑PLi/3 | • | • | • | • | • | • | • |  |
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| IS.3153‑PLi/3 | • | • | • | • | • | • | • |  |
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| IS.3152‑PLFi/3 | • | • | • | • | • | • | • | |||||
| IS.3153‑PLFi/3 | • | • | • | • | • | • | • |
1-position slider attachment
Blue: single position slider fluorescence attachment with a 3 W NeoLED™ light source for blue excitation (/LB)
Green: single position slider fluorescence attachment with a 3 W NeoLED™ light source green excitation (/LG)
| MODELS | Binocular | Trinocular | WF 10x/22 mm | Diopter adjustable on left tube | Diopter adjustable on both tubes | E-plan EPLi 4x/10x/S40x and S100x objectives | Plan PLi 4x/10x/S40x and 100x objectives | Plan PLFi 4x/10x/S40x and S100x objectives | Blue excitation 460-495 nm | Green excitation 520-530 nm | NeoLED illumination | |
| IS.3152‑EPLi/LB | • | • | • | • | • | • | ||||||
| IS.3153‑EPLi/LB | • | • | • | • | • | • | ||||||
| IS.3152‑PLi/LB | • | • | • | • | • | • | ||||||
| IS.3153‑PLi/LB | • | • | • | • | • | • |  |
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| IS.3152‑PLFi/LB | • | • | • | • | • | • | ||||||
| IS.3153‑PLFi/LB | • | • | • | • | • | • | ||||||
| IS.3152‑EPLi/LG | • | • | • | • | • | • | ||||||
| IS.3153‑EPLi/LG | • | • | • | • | • | • | ||||||
| IS.3152‑PLi/LG | • | • | • | • | • | • | ||||||
| IS.3153‑PLi/LG | • | • | • | • | • | • |  |
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| IS.3152‑PLFi/LG | • | • | • | • | • | • | ||||||
| IS.3153‑PLFi/LG | • | • | • | • | • | • |
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EYEPIECES
Extended EWF 10x/22 mm eyepieces, Ø 30 mm tubes. Eyepieces can be secured to the tubes. The trinocular head of the Plan infinity corrected models (PLi and PLFi) has a light path selector (100:0 / 50:50) and ± 5 diopter adjustments on both tubes.
The trinocular head of the E-Plan infinity corrected models (EPLi) has a fixed built-in light path beamsplitter (50:50) and ± 5 diopter adjustment on the left tube
HEAD
• Binocular and trinocular Siedentopf type heads with 30° inclined tubes. Interpupillary distance from 48 to 76 mm
• A unique rotating system allows the ergonomical positioning of both tubes in a high (431 mm) and in a low position (397 mm)
• The trinocular head has a 23.2 mm photo tube. This fits all C-mount adapters supplied with Euromex cameras
To order an iScope® with tilting head use reference IS.3158-xxx (Epli/Pli/PLFi)
To order an iScope® with tilting trino head use reference IS.3159-xxx (EPLi/Pli/PLFi)
NOSEPIECE
Revolving quintuple reversed nosepiece on ball bearings
OBJECTIVES
The EPLi and PLi objectives are suitable for Blue and Green standard filter sets and other filter sets in the visible spectrum.
The plan Fluarex PLFi ™ objectives are suitable for the entire spectrum including Ultra Violet and Violet
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E-Plan EPLi IOS |
4x/0.10 |
10x/0.25 |
S40x/0.65 |
S100x/1.25 oil |
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Plan PLi IOS |
4x/0.10 |
10x/0.25 |
20x/0.40* |
S40x/0.65 |
S60x/0.85* |
S100x/1.25 oil |
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Plan PLFi IOS |
4x/0.15 |
10x/0.35 |
20x/0.60* |
S40x/0.75 |
S100x/1.30 oil |
* optional
All optics are anti-fungus treated and anti-reflection coated for maximum light throughput
STAGE
187/230 x 140 mm rackless stage equipped with double slide holder and integrated 79 x 52 mm mechanical stage. The iScope® rackless stage has no protruding parts, enabling more smooth movements and is safer
Optional: 220 x 157 mm ceramic stage with integrated 75 x 50 mm mechanical stage protects with a very tough ceramic surface, against marks and scratches. The surface also ensures stray light not to be reflected from the stage into the objective
CONDENSER FOR BRIGHTFIELD
The standard height adjustable Abbe N.A. 1.25 condenser for brightfield with magnification indication.
Also accepts sliders with phase annuli suitable for plan phase 10x/20x or S40x/ S100x oil immersion IOS objectives for economical phase contrast. A slider with a simple dark stop for darkfield up to 400x magnification is also available for this condensor
CONDENSER FOR ZERNIKE PHASE CONTRAST
An optional height adjustable Zernike NA. 1.25 phase contrast disc condenser with darkfield stop position for darkfield contrast observation up to 400x magnification and plan phase 10/20/S40x and S100x oil immersion PLPHi IOS objectives
FOCUSING
Coaxial coarse and fine adjustments, 200 graduations, 1 μm per graduation, 200 μm per rotation, total travel range is approximately 24 mm. Supplied with an adjustable rack stop to prevent damage to sample and objectives. The coarse adjustments are equipped with friction control
ILLUMINATION
3 W adjustable Köhler NeoLED diascopic illumination with internal 100-240 V power supply
The innovative NeoLED design offers larger apertures, allowing the optical system of the iScope microscope to produce images at higher resolutions, very close to the theoretical diffraction limit of the optics. Other benefits of the NeoLED is the low energy consumption, no heating and a long operating lifetime
The Köhler illumination on models with IOS plan and plan phase infinity corrected system provides high contrast and a maximum achievable resolving power of the optics. Also available for other models as an option
ILLUMINATION FOR FLUORESCENCE
The 6-position rotating and 3-position slider fluorescence attachments are supplied with:
• a 100 W HBO mercury vapor light source for broad powerful light spectrum. Including an 100-240 V external power supply with indication of the life span of the HBO bulb
• Standard fluorescence filters sets for
- blue excitation (EX460- 490 (BP) DM520 EM500 (LP)
- green excitation (EX510-550 (BP) DM580 EM570 (LP) and a UV protection shield
The iScope® fluorescence microscopes equipped with a 100 W mercury vapor light source can be supplied with more specific, optimized and application related fluorescence filters sets (see next pages).
Two single position LED fluorescence attachments:
- Blue: EX 460-495 DM 505 EM 510-560 LP filter set and a 3 W 465-475 nm LED for blue excitation
- Green: EX 510-550 DM570 EM 590 LP filter set and a 3 W 520-530 nm LED light source for green excitation
Both LED versions are supplied with a 100-240 Vac/7.5 Vdc external power supply
STANDARD GENERAL PURPOSE FLUORESCENCE FILTER SETS
IS.9745 - Blue excitation filter set
EX: 460-490 nm bandpass DM: 520 nm EM: 500 nm longpass
IS.9746 - Green excitation filter set
EX: 510-550 nm bandpass DM: 580 nm EM: 570 nm longpass
IS.9747 - UV excitation filter set
EX: 330-385 nm bandpass DM: 420 nm EM: 400 nm longpass
IS.9748 - Violet excitation filter set
EX 400-410 nm bandpass DM 455 nm EM 455 nm longpass
ICARE SENSOR
The unique iCare Sensor is developed to avoid unnecessary loss of energy. The illumination of the microscope automatically switches off shortly after microscopists step away from their position
CSS - CABLE STORAGE SYSTEM
iScope allows users to easily insert the power cable into the back of the instrument, which enables easy storage. The integrated carrying grip at the back of the microscope ensures safe transportation of the microscope
CONFIGURATIONS
The iScope microscope is available in many configurations and can be upgraded with a variety of contrast types
PACKAGE CONTENT
Supplied with power cord, dust cover, a spare fuse, user manual and 5 ml immersion oil for models with S100x objective. Models with 100 W mercury vapor light source have a UV protection shield. All packed in polystyrene boxes
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PB.5276 Microscoop-onderhoudsset, 16 onderdelen: schoonmaakpenseel, 6-delige schroevendraaiersset, blaasbalgje, 3-delige inbusset (1,5, 2, 2,5 mm), lensreinigingsvloeistof 20 ml, reinigingsdoek 140 x 140 mm, 100 stuks lenstissues, tube onderhoudsvet, 10 ml fles olie, verpakt in een overzichtelijke opbergdoos |
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PB.5157-B Objectglaasjes 76 x 26 mm, wit glas, kanten geslepen. Een kant blauw. Per 50 stuks verpakt |
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PB.5157-W Objectglaasjes 76 x 26 mm, wit glas, kanten geslepen. Per 50 stuks verpakt |
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IS.5700 Ergonomische kantelbare kop 5 tot 35°. Voor iScope oneindig gecorrigeerde optische systemen |
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IS.6010 EWF 10x/20 mm oculair, Ø 23,2 mm tubus |
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IS.6010-C EWF 10x/20 mm oculair met kruisdraad, Ø 23,2 mm tubus |
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IS.6010-P EWF 10x/20 mm oculair met aanwijsnaald, Ø 23.2 mm tubus |
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IS.6010-CM EWF 10x/20 mm oculair met 10/100 micrometer en kruisdraad, Ø 23,2 mm tubus |
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IS.6015 WF 15x/16 mm oculair, Ø 23,2 mm tubus |
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IS.6020 WF 20x/12 mm oculair, Ø 23,2 mm tubus |
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IS.6099 Paar oogschelpen, Ø 23,2 mm tubus |
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IS.6210 EWF 10x/22 mm oculair, Ø 30 mm tubus |
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IS.6210-C EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus |
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IS.6210-P EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus |
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IS.6210-CM EWF 10x/22 mm oculair met 10/100 micrometer en kruisdraad, Ø 30 mm tubus |
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IS.6212 WF 12,5x/15 mm oculair, Ø 30 mm tubus |
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IS.6215 WF 15x/16 mm oculair, Ø 30 mm tubus |
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IS.6220 WF 20x/12 mm oculair, Ø 30 mm tubus |
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IS.6299 Paar oogschelpen, Ø 30 mm tubus |
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IS.7104 E-plan EPL 4x/0,10 objectief |
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IS.7110 E-plan EPL 10x/0,25 objectief |
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IS.7120 E-plan EPL 20x/0,40 objectief |
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IS.7140 E-plan EPL S40x/0,65 objectief |
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IS.7160 E-plan EPL S60x/0,85 objectief |
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IS.7100 E-plan EPL S100x/1,25 objectief |
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IS.7710 Plan PLPH 10x/0,25 fasecontrast objectief |
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IS.7720 Plan PLPH 20x/0,40 fasecontrast objectief |
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IS.7740 Plan PLPH S40x/0,65 fasecontrast objectief |
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IS.7700 Plan PLPH S100x/1,25 fasecontrast objectief |
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IS.8804 E-plan EPLi 4x/0,10 IOS oneindig gecorrigeerd objectief |
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IS.8810 E-plan EPLi 10x/0,25 IOS oneindig gecorrigeerd objectief |
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IS.8840 E-plan EPLi S40x/0,65 IOS oneindig gecorrigeerd objectief |
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IS.8800 E-plan EPLi S100x/1,25 IOS oneindig gecorrigeerd objectief |
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IS.7202 Plan PLi 2x/0.05 IOS oneindig gecorrigeerd objectief, geen dekglascorrectie. Werkafstand 18,3mm |
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IS.7204 Plan PLi 4x/0,10 IOS oneindig gecorrigeerd objectief |
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IS.7210 Plan PLi 10x/0,25 IOS oneindig gecorrigeerd objectief |
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IS.7220 Plan PLi 20x/0,40 IOS oneindig gecorrigeerd objectief |
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IS.7240 Plan PLi S40x/0,65 IOS oneindig gecorrigeerd objectief |
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IS.7260 Plan PLi S60x/0,85 IOS oneindig gecorrigeerd objectief |
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IS.7200 Plan PLi S100x/1,25 IOS oneindig gecorrigeerd objectief |
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IS.7200-I Plan PLi S100x/1,25 IOS oneindig gecorrigeerd objectief met geïntergreerde irisdiafragma |
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IS.8910 Plan PLPHi 10x/0,25 fasecontrast IOS oneindig gecorrigeerd objectief |
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IS.8920 Plan PLPHi 20x/0,40 fasecontrast IOS oneindig gecorrigeerd objectief |
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